Primary Human Nasal Epithelial Cell Culture.

Mystica Terrance, Tarini Gunawardena,Hong Ouyang,Julie Avolio,Wenming Duan, Sowmya Thanikachalam,Theo J Moraes

Methods in molecular biology (Clifton, N.J.)（2024）

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摘要

Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the respiratory tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.

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epithelial,cell

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