Protective effects and microarray-based mechanism of sea cucumber hydrolysates against high-glucose induced nephrotoxicity in mouse glomerulus mesangial cells

Diabetic nephropathy (DN) is a common type of end-stage renal disease and glomerular mesangial cells Diabetic nphropathy (DN) is a comon type of end-tage renal diseas and glomular mesangial cells (GMCs) are widely used as a cell model for DN. This study firstly inve stigated the inhibitory effects of the (GMCs) Apostichopusare widely japonicusused as aand cell Acaudinamodel for DNleucoproctaThis studyhydrolysates firstly o cllulrinvestigated growththe underinhibitory high-glucoseeffects of htreatmen,Apostichopusbetter inhibitoryjaponicus effectand ofA.Acaudina japonics leucoproctahydrolysate hydrolyatewas observedon cellularcompared growthto that underofA. high-glucoseleucoprocta treatment,hydrolysate. better Subsequentl,inhibitory theeffect glbalofA. jponcustranscription profileshydrolysate wasobtained viaobserved micrarraycompared toanalysis that of showedA. thatleucoprocta6 070 hyrolysae. Subsequently, the global transcription profiles obtained via microarray nalyss showed tha 6 070 and 7 015 genes were identified in the A. japonicus and A. leucoprocta groups compared with the model group, respectively. Among them, transcriptions f the slc30a4, slc35d1, tppp3, tp53inp1, bcl-2, apaf1, alox12b and 7 015 genes were identified in the A. japonicus and A. leucoproct group compared with the model group, and respectively. adrala genes Among were thm, restored from transcripions the levels of the of the slc30a4, model s groupc35d1 to tppp3,those of thetp53inp1, controbcl 2, group, apaf1, contributed alox12b to and cell adrala mitosis genes and wee prolifration restored infrom bothe lvelstreatment of the groups.model In groupaddition, to thosother of the apoptosis-eatedcontrol group, genes, contribusuch edas tobcl-6,cel clu, mitosis foxo3 and and prolife aktl, atinshowed in bothopposit treamenttrends groups.between In two additin,groups, otherwhich mightapoptosis-related cause the genes, differenesuch asin inhibitory effect. We preiminarily proposd that the regulation effects of A. japonicus nd A. leucoprocta o bcl-6, clu foxo3 and aktl, showed opposite rends between two groups, wh ch might cause the difference in the genes involved in clular mtosis, roliferation and apoptsis, might contribute to their inhibitry aivity inhbitory effect. We preliminarily proposed that the regulation effects of A. japonicus and A. leucoprocta on the gene involved in cellular mitosis, proli on GMCs under high-glucose environment. . .. (c) 2024 Beijing Academy of Food Science. Publishing services by Elsevier B.V on behalf of KeAi Communications Co Ltd This is an open access artic e under the CC BY-NC-ND license ., . r-- cense Communications Co , Ltd This is an open acss article under the CC BY NC ND license (http://creativecommons org/licenses/by nc nd/4 0/) (http://creatvecommons.org/censes/by-nc-nd/4.0/). (http://creativecommons org/licenses/by nc nd/40/)