Demethylation-Driven ligase chain reaction for simultaneously sensitive detection of m6A demethylase FTO and m1A demethylase ALKBH3 in breast cancer at Single-Molecule level

Ming-hao Liu,Qian Zhang,Wan-tong Yu,Jian-Ge Qiu,Hui-min Yuan,Chuan-rui Wang,Bing-Hua Jiang,Yan Zhang,Chun-yang Zhang

Chemical Engineering Journal（2023）

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摘要

•We demonstrate simultaneous single-molecule detection of FTO and ALKBH3.•Only a single DNA ligase can achieve multi-cyclic cascade amplification.•This method exhibits high sensitivity and good specificity.•This method can screen inhibitors and detect cellular RNA demethylases.•This method can discriminate breast cancer patients from healthy persons.

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关键词

m6a demethylase fto,demethylase alkbh3,ligase chain reaction,breast cancer,demethylation-driven,single-molecule

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