We present a genome assembly from an individual female Hesperia comma (the Silver-spotted Skipper; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The genome sequence is 525.3 megabases in span. Most of the assembly is scaffolded into 29 chromosomal pseudomolecules, including the Z and W sex chromosomes. The mitochondrial genome has also been assembled and is 17.73 kilobases in length. Gene annotation of this assembly on Ensembl identified 18,725 protein coding genes.
