Intrinsic peroxidase-like clay mineral nanozyme-triggered cascade bioplatform with enhanced catalytic performance

Recently, the combination of nature enzymes and nanozymes provides new opportunities for constructing new generation of high-performance enzymatic cascade system. Herein, an enzyme cascade bioplatform (GOx-BSA/ NAu-2) has been constructed by combining clay mineral nanozymes (nontronite, NAu-2) and natural enzyme (glucose oxidase, GOx). NAu-2 can be used both as a carrier for GOx immobilization and as a mimic of peroxidase (POD) guiding an enzyme cascade bioplatform. Owing to the stabilizing effect of bovine serum albumin (BSA) on enzymes and proper immobilization method, the GOx-BSA/NAu-2 possess better thermal stability, acid-base stability, storage stability and reusability than free enzymes system. Given the substrate channel effect, the acquired GOx-BSA/NAu-2 exhibits higher catalytic efficiency than the BSA/NAu-2 system and the GOx + HRP system. This work provides an efficient and simple reference for the design of clay mineral nanozyme-enzyme bioplatform, and also broadens the application field of clay minerals.