Roseateles amylovorans sp. nov., isolated from freshwater

A Gram-stain-negative, rod-shaped, amylolytic bacterial strain, designated as bsSlp3-1(T), was isolated from the Slepian water system, a freshwater reservoir. Strain bsSlp3-1(T) was found to be aerobic, oxidase-positive and catalase-negative, grew at 5-37 degrees C (optimum, 28 degrees C), pH 5.0-9.5 (optimum, pH 7.0) and low NaCl concentration (up to 1.0 %). Comparative analysis of 16S rRNA gene sequence similarity revealed that strain bsSlp3-1(T) clustered with Roseateles species and is closely related to Roseateles depolymerans KCTC 42856(T) (98.7%) and Roseateles terrae CCUG 52222(T) (98.6%). Whole-genome comparisons using average nucleotide identity and digital DNA-DNA hybridization values suggested that strain bsSlp3-1(T) represents a novel species within the genus Roseateles and is most closely related to Roseateles aquatilis CCUG 48205(T) (81.2 and 25.6%, respectively). The genome of strain bsSlp3-1(T) consisted of a single circular chromosome with size 6289366 bp and DNA G+C content of 66.8 mol%. The predominant cellular fatty acids of bsSlp3-1(T) were cis-9-hexadecanoic and hexadecenoic acids. According to the data obtained in this work, strain bsSlp3-1(T) represents a novel Roseateles species for which the name Roseateles amylovorans sp. nov. is proposed. The type strain is bsSlp3-1(T) (=BIM B-1768(T)= NBIMCC 9098(T)=VKM B-3671(T)).