JEasyTFM: an open-source software package for the analysis of large 2D TFM data within ImageJ

Motivation Cells adhering to the extracellular matrix can sense and respond to a wide variety of chemical and physical features of the adhesive surface. Traction force microscopy (TFM) allows determining the tensile forces exerted by the cells on their substrate with high resolution.Results To allow broad access of this techniques to cell biology laboratories we developed JeasyTFM, an open-source ImageJ package able to process multi-color and multi-position time-lapse pictures thus suitable for the automatic analysis of large TFM data.Availability and implementation JEasyTFM is implemented as an ImageJ plugin and available at: http://questpharma.u-strasbg.fr/JEasyTFM.html.