Vagus nerve mediated liver-brain axis is a major regulator of the metabolic landscape in the liver
biorxiv(2023)
摘要
Background The liver serves as a major energetic reservoir for other tissues and its metabolic function is controlled by humoral and neural factors. The vagus nerve innervating the gastrointestinal tract plays an important role in regulating peripheral metabolism and energy expenditure. Although the liver receives vagus nerve fibers, the impact of this circuitry in the regulation of hepatic metabolism is still poorly understood.
Methods Herein, we used a combination of quantitative proteomics and in vivo imaging techniques to investigate the impact of the vagus nerve on liver metabolism.
Results Vagus nerve shapes the metabolic framework of the liver, as surgical ablation (vagotomy; VNX) of this circuitry led to a significant alteration of the hepatic proteome landscape. Differential protein expression and pathway enrichment analyses showed that glycolytic and fatty acid biosynthesis were increased following VNX, whereas β-oxidation was decreased. This metabolic shift facilitated lipid accumulation in hepatocytes. Furthermore, VNX worsened liver steatosis following high-carbohydrate or high-fat dietary challenges.
Conclusions This study describes the liver-brain axis mediated by the vagus nerve as an important regulator of the hepatic metabolic landscape.
Highlights
### Competing Interest Statement
The authors have declared no competing interest.
* ZT
: Zeitgeber Time
TEAB
: Triethylammonium Bicarbonate
TCEP
: Tris(2-carboxyethyl)phosphine
Hz
: Hertz
ACN
: Acetonitrile
TFA
: Trifluoroacetic Acid
VNX
: Vagotomy
MS
: Mass Spectometry
HCD
: High-carbohydrate diet
HFD
: High-fat diet
KEGG
: Kyoto Encyclopedia of Genes and Genomes
TCA
: Tricarboxylic Acid Cycle
DMSO
: dimethyl sulfoxide
Gck
: Glucokinase
Pfkl
: Phosphofructokinase
Aldob
: Aldolase, fructose-bisphosphate B
Pklr
: Pyruvate kinase liver and red blood cell
Fasn
: Fatty acid synthase
Acaca
: Acetyl-Coenzyme A carboxylase alpha
Acacb
: Acetyl-Coenzyme A carboxylase beta
Mcat
: Malonyl CoA:ACP acyltransferase (mitochondrial)
Scd1
: Stearoyl-Coenzyme A desaturase 1
Acot1
: Acyl-CoA thioesterase 1
Hsd17b12
: hydroxysteroid (17-beta) dehydrogenase 12
Cyp4a14
: Cytochrome P450, family 4, subfamily a, polypeptide 14
Acox1
: Acyl-Coenzyme A oxidase 1, palmitoyl
Acaa1a
: Acetyl-Coenzyme A acyltransferase 1A
Fbp1
: Fructose bisphosphatase 1
Pck1
: Phosphoenolpyruvate carboxykinase 1, cytosolic
NAFLD
: Non-Alcoholic Fatty Liver Disease
NASH
: Non-Alcoholic Steatohepatitis
NTS
: Nucleus of the solitary tract
DMN
: Dorsal motor nucleus of the vagus
NA
: Ambiguus nucleus
PCA
: Principal Component Analysis
FDR
: False Discovery Rate
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