High-Speed TIRF and 3D Super-Resolution Structured Illumination Microscope with Large Field of View Based on Fiber Optic Components

Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fastest and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but the cost of the instruments is still relatively high and the physical size of the instruments is still prohibitively large. We have developed a compact, cost-efficient and high-speed fiber-based 2D-, grazing-incidence-, TIRF and 3D-SIM to overcome these limitations [1], [2]. The fiber-based illumination path allows multi-color imaging with a field-of-view (FOV) of up to $150\times 150\upmu\mathrm{m}^{2}$ . The setup can be extended with a real-time-reconstruction to immediately assess the image quality.