FIGURE 3 from Surgical Inflammation Alters Immune Response to Intraoperative Photodynamic Therapy

TI does not significantly impede direct PDT damage. Clonogenic analysis of tumor tissue immediately (A) or 24 hours (B) after PDT. At 24 hours after TI and/or PDT, reductions were observed in the median number of clonogenic cells per gram of tumor tissue compared with untreated controls (9.3-fold change for TI, 5.9-fold change for PDT, and 4.9-fold change for TI/PDT). Significance was only observed in the TI/PDT group versus untreated tumor control (TC, P = 0.0192). C, Immunoblot analysis of PARP cleavage 24 hours after PDT administration revealed no differences in TI-exposed tumors. n = 4 mice per group. D, Histologic samples taken from TI-, PDT-, or TI/PDT-treated tumors. H&E-stained sections are shown at 10X and 20X magnification. One representative image is shown from three repetitions showing similar extent of necrosis between PDT- and TI/PDT-treated tumors. n = 7–11 mice per group.