Toxoplasmosis Outbreak Caused by North American Genotypes in Captive Black-Tufted Marmosets in Southern Brazil

In this study, we describe an outbreak of toxoplasmosis in an enclosure of five Callithrix penicillata in Southern Brazil. The marmosets were found dead and submitted to necropsy and histopathology. Liver, spleen, and lungs were frozen and sent for polymerase chain reaction (PCR) and nested-PCR-restriction fragment length polymorphism (RFLP) genotyping of Toxoplasma gondii. Necropsy revealed a liver with a lobular pattern, along with diffusely congested lungs, spleen, and kidneys, and hemorrhage in the mesenteric lymph nodes. Mild-to-moderate, multifocal, necrotic hepatitis and splenitis, multifocal lymphoplasmacytic myocarditis, and moderate, diffuse necrohemorrhagic lymphadenitis were observed. T. gondii tachyzoites were in all the organs mentioned. The detected strains were closely related with the isolates TgWtdUs10, TgSoUs39, and TgShUs2, which were originally found in non-primate species all in the United States of America. This is an unprecedented report of North American strains causing mortality in captive individuals of the species Callithrix penicillata in the Southern Brazil.