Vinyl Sulfone Functionalized Magnetic Nanoparticles and Its Application in Palmitoylated Peptides Enrichment

Vinyl sulfone modified magnetic nanoparticles(denoted as MagBVP) were synthesized and used as the magnetic carrier of magnetic solid phase extraction(MSPE) platform, combined with MALDI-TOF MS or LC-MS/MS. The MagBVP was characterized by means of vibrating sample magnetometer, field emission scanning electron microscopy, infrared spectroscopy, zeta potential measurement and X-ray diffraction. The synthesized palmitoylated palmitoyl-coA was selected as the modeling palmitoylated peptide sample. Under the optimal conditions, the MSPE- MS platform could achieve high selectivity(BSA tryptic digests/palmitoylated peptide molar ratio of 2000:1) and sensitivity(the detection limit was determined to be as low as 1.0 fmol/mu L) for palmitoylated peptide. Finally, the method was applied to the determination of palmitoylated peptides in cell lysates samples. These results showed that the magnetic material modified by vinyl sulfone had a high selectivity for palmitoylomics.