Substrate specificity and inhibition studies of human serotonin N-acetyltransferase.

Throughout the text, all of theVmax units read “μmol/min/mg protein,” whereas they should read “nmol/min/mg protein.” This mistake is especially evident in the following occurrences. Page 8797, right column: The next to last sentence in the last paragraph should read : “Comparison of the data obtained with this HPLC assay with those generated with the extraction assay showed similar activities (not shown) agreeing at least within a factor 2: 1.33 and 1.66 nmol/min/mg protein for activities measured with the fused hAANAT and 16.6 and 33.3 nmol/min/mg protein for the pure hAANAT.” Page 8799, left column: The 11th line from the bottom of the page should read: “enzyme with aVmax of 438 ± 27 nmol/min/mg of protein.” Page 8799, right column: The 10th through 14th lines should read: “Nevertheless, none of the alternate synthetic substrates had better rate constants than PEA (∼4000 nmol/min/mg protein) or tryptamine (∼800 nmol/min/mg protein), whereas for serotonin, it was comparable to the aryl bicyclic compounds (∼200–300 nmol/min/mg protein).” This correction should also be made in the unit headings of Tables II and III (pages 8799 and 8800, respectively).