Expression of transferrin receptor 1, proliferating cell nuclear antigen, p27Kip1 and calbindin in the fetal and neonatal feline cerebellar cortex

Cerebellar cortices from feline fetuses with estimated gestational ages of 40-66days and from kittens aged 2days to 2months, all negative for feline panleukopenia virus (FPV) infection, were analysed for expression of the transferrin receptor 1 (TrFR1), proliferating cell nuclear antigen (PCNA), p27(Kip1) and calbindin. TrFR1, the receptor used by FPV to enter target cells, was expressed in capillary endothelial cells in the cerebellum at all fetal stages investigated and in Purkinje cells of a 3-week-old kitten, but not in the neuroblasts in the external granule layer (EGL). PCNA was expressed in cells of the superficial layer of the EGL. The cyclin-dependent kinase inhibitor p27(Kip1) was expressed in cells of the deep layer of the EGL. Purkinje cells expressed calbindin from the earliest fetal stage investigated. Co-expression of PCNA and calbindin could not be demonstrated, indicating that feline Purkinje cells are post-mitotic from at least 40days gestation.