Suppression of lipopolysaccharide-induced COX-2 expression via p38MAPK, JNK, and C/EBP phosphorylation inhibition by furomagydarin A, a benzofuran glycoside from Magydaris pastinacea
Journal of Enzyme Inhibition and Medicinal Chemistry(2024)
摘要
The phytochemical investigation of the methanol extract of the seeds of Magydaris pastinacea afforded two undescribed benzofuran glycosides, furomagydarins A-B (1, 2), together with three known coumarins. The structures of the new isolates were elucidated after extensive 1D and 2D NMR experiments as well as HR MS. Compound 1 was able to inhibit the COX-2 expression in RAW264.7 macrophages exposed to lipopolysaccharide, a pro-inflammatory stimulus. RT-qPCR and luciferase reporter assays suggested that compound 1 reduces COX-2 expression at the transcriptional level. Further studies highlighted the capability of compound 1 to suppress the LPS-induced p38MAPK, JNK, and C/EBP beta phosphorylation, leading to COX-2 down-regulation in RAW264.7 macrophages.
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关键词
COX-2,benzofurans,Magydaris pastinacea,p38MAPK,JNK,C/EBP beta
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