Novel bioinspired dinuclear Cu(II) 'paddle wheel' acetate complex: Catalytic and in vitro biological activity studies

A versatile bioinspired metallocatalyst [Cu2L2(OAc)4] (L = 2-(3-(dimethylamino)propyl)isoindoline-1,3-dione) has been synthesized and found distorted octahedral complex on the basis of various spectroscopic characterization. XRD date shows that it is monoclinic system with space group C 2/c. Non classical intramolecular Hbond stabilise it's dimeric nature. The additional signal appeared at g ca 2.10 in EPR and shifting of irreversible cathodic peak from -0.292 V to -0.910 V in CV confirm it is phenoxazinone synthase activity while base peak at m/z 221.15 obtained from ESI Mass and irreversible cathodic peak shifting from -0.292 to -0.211 V ratify it's catechol oxidase activity. Besides, Michaelis-Menten analysis shows prominent catalytic activity towards 3,5DTBC and 2-AP (Kcat 12.0726x103 h-1 and 6.6654x103 h-1). In vitro study supported by molecular docking study exposes substantial dose dependent anti-leukemic activity against Hep-G2 cell line (GI50 < 10 mu g/mL). Moreover, promising anti-bacterial property was also detected on multi-drug resistant E. coli and B. cereus bacteria (B. cereus OD 0.21 +/- 0.016; E. coli OD 0.172 +/- 0.013) at 100mM concentration of complex.