FIGURE 5 from Integrin-αvβ3 is a Therapeutically Targetable Fundamental Factor in Medulloblastoma Tumorigenicity and Radioresistance

In vivo measurement of integrin-αvβ3 expression using SPECT/MRI dual-modality. A, Representative coronal views of MRI (bottom), SPECT (center), and fused SPECT/CT/MRI of HD-MB03 (left) and DAOY (right) tumors at 1 hour postinjection of ^99mTc-RAFT-RGD. B,In vivo quantification of ^99mTc-RAFT-RGD tumor uptake from SPECT images. Results are expressed as tumor-to-cerebellum ratios. C, Autoradiography on 20 µm tumor-containing cerebellum slices (top) and staining of adjacent slices by Crystal Violet. D, Autoradiographic image quantification of ^99mTc-RAFT-RGD uptake in HD-MB03 and DAOY tumor lesions. Results are expressed as tumor-to-cerebellum ratios. Key: *, P < 0.05; **, P < 0.01 versus HD-MB03.