miR-205-5p Carried by Lipid Nanoparticles Regulates PI3K/AKT Pathway of Treg Cells in the Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma and Down-Regulates PD-L1

Laryngeal squamous cell carcinoma (LSCC) is the main disease type in laryngeal cancer. With the increasing number of patients with laryngeal cancer, the number of patients with LSCC is also increasing. miR-205-5p participates in the development of tumor cells. This study mainly explores how miR-205-5p carried by lipid nanoparticles regulates Treg cells in the tumor microenvironment of LSCC and affects PI3K/AKT pathway to down-regulate PD-L1. Lipid nanoparticles loaded with miR-205-5p and a rat model was constructd followed by analysis of CD25 + and Treg cells by flow P: 203 8 109 20 On: Tue 05 Sep 2023 06:27 50 cytometry, morphology of tumor tissue by HE staining, Treg cells apoptosis and the expression of PI3K, AKT, Nrf2, and Copyright: American Scientific Publishers PD-L1 by qRT-PCR and Western blot. The nanoparDelivredticles werebyuniforngentamly dispersed and the surface was smooth. The particle size distribution was good. Tumors were formed in the right axilla of nude mice and the tumor cells were divided into three grades. Lipid nanoparticles loaded with miR-205-5p induced apoptosis of Treg cells. The apoptosis of Treg cells was related to PI3K/AKT signaling, apoptosis was activated, and the level of Nrf2 decreased after treatment with PI3K/AKT inhibitors. Nrf2 down-regulated the expression of PD-L1 and induced Treg cell apoptosis. miR-205-5p-loaded lipid nanoparticles inhibited the PI3K/AKT signaling pathway, activated Nrf2, reduced PD-L1 expression, and promoted the apoptosis of Treg cells. miR-205-5p carried by lipid nanoparticles can inhibit PI3K/AKT signaling pathway, activate Nrf2, and down-regulate PD-L1, thereby accelerating the apoptosis of Treg cells in the tumor microenvironment of LSCC.