IDDF2023-ABS-0231 The study of clinical characteristics and intestinal microbiota of pediatric clostridiodes difficile infection

Background

To evaluate the clinical characteristics, microbial composition, and gut metabolites of Clostridiodes difficile infection (CDI) in children.

Methods

91 stool samples of CDI patients from September 2014 to August 2022 were collected, including 30 RCDI and 61 non-RCDI. 16S rRNA sequencing and liquid chromatography/mass-spectrometry metabolomics were used to analyze the microbiota composition and metabolites in children with CDI.

Results

The original patients’ microbiota had low diversity in RCDI patients. Fecal samples of RCDI patients were rich in members of Lactobacillales, Bacilli, Entotheonellales, Reyranellas, Leuconostocaceae, Erwinia and so on. The most significantly increased in the CDI group were Lachnospiraceae, Erysipelotrichi, Erysipelotrichaceae, Erysipelotrichales, Ruminococcus, Ruminococcaceae and so on. A total of 37 potential stool biomarkers were screened out in this study, among which the five most significantly different were arachidonic acid, 3−Hydroxyisovalerylcarnitine, γ-linolenic acid, Eicosapentaenoic acid (EPA), α-linolenic acid.

Conclusions

Some functional strains are altered in RCDI children. Compared with non-RCDI patients, some lipid metabolites changed in RCDI.