Inhibitory Effect of the Darae Pollen ( Actinidia arguta) Extract on the Growth of LNCaP Human Prostate Cancer Cell Line

Bee pollen is collected in the form of dumplings by gathering nectar and enzymes on their hind legs when bees collect nectar from flowers. Recently, nutrients in bee pollen have been reported that the nutrients are effective for various diseases such as prostate enlargement and cancer. In this study, the anti-prostate cancer effect was investigated by selecting pollen from the most research reports, darae pollen ( Actinidia arguta). In order to confirm the purity of the pollen, it was observed using a microscope and a scanning electron microscope (SEM), and the identification of the pollen was confirmed the darae pollen ( Actinidia arguta 85%, Amorpha spp. 14%) through genetic analysis. Except for Bax among the apoptosis related proteins increased in the expression level of the pollen extract. AIF was also increased, which seems to have led to the initiation of apoptosis due to the release of AIF in the mitochondria into the cytosol. Akt, a protein involved in promoting cell division, decreased and the amount of BCL-2 protein also decreased according to pollen extract concentration. In conclusion, it was confirmed that the apoptosis related protein increased and the cell division promoting protein decreased, according to this result, the darae pollen extract was effective against prostatic hyperplasia or prostate cancer.