Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing
Smart Materials in Medicine(2023)
摘要
Bone repair processes are tightly affected by fiber topographies of scaffolds and can be promoted by coupling with chemotactic and/or angiogenic molecules. Here, we developed polycaprolactone (PCL) and collagen-based fibrous scaffolds expressing various architectures via a modified electrospinning set up. We conjugated the as-spun scaffolds with caffeic acid (CA) and/or a cartilage oligomeric matrix protein of angiopoietin 1 (COMP-Ang1). The CA-coupled PCL/collagen scaffold (PCL/col/CA) exhibited greater treatment efficacies for biomimetic and cellular mineralization, expression of osteogenic and chemotactic molecules, and cell migration than did the PCL/col treatment alone. Among the PCL/col/CA scaffolds, the radially symmetric grid-patterned scaffold (rG-PCL/col/CA) showed the greatest bioactivities. The linking of the rG-PCL/col/CA with COMP-Ang1 increased the expression of vascular endothelial growth factor by cells. The COMP-Ang1-linked rG-PCL/col/CA formed more new blood vessels and expressed more chemotactic molecules in a rat model of femoral defects than did the scaffold alone. Compared with PCL/col/CA scaffolds, the COMP-Ang1-coupled rG-PCL/col/CA scaffold stimulated faster and greater healing of femoral defects. Collectively, this study demonstrates that the coupling of a radially grid-patterned fibrous scaffold with CA and COMP-Ang1 greatly enhances scaffold-mediated bone healing via synergistic improvements in vascularization, cell migration, and formation and maturation of new bones in defected regions.
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关键词
Radially grid-patterned fibrous electrospun scaffold,COMP-Ang1,Caffeic acid,Neovascularization,Guided bone repair
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