Distinct microbial taxa are associated with LDL-cholesterol reduction after 12 weeks of Lactobacillus plantarum intake in mild hypercholesterolemia

Abstract Probiotic microbes such as Lactobacillus may reduce serum total cholesterol (TC) and low-density lipoprotein (LDL) cholesterol. The objective of this study was to assess the effect of Lactobacillus plantarum strains CECT7527, CECT7528 and CECT7529 (LP) on the serum lipids, cardiovascular parameters and fecal gut microbiota composition in patients with mild hypercholesterolemia. A randomized, double-blinded, placebo-controlled clinical trial with 86 healthy adult participants with untreated elevated LDL cholesterol ≥ 160 mg/dL was conducted. Participants were randomly allocated to either placebo or LP (1.2 x10 9 CFU/d) for 12 weeks. LDL, HDL, TC and triglycerides (TG), cardiovascular parameters (blood pressure, arterial stiffness) and fecal gut microbiota composition (16S rRNA gene sequencing) were assessed at baseline and after 12 weeks. Both groups were comparable regarding age, sex and LDL-C at baseline. LDL-C decreased (mean decrease − 6.6 mg/dl ± -14.0 mg/dl, P time*intervention = 0.006) in the LP group but not in the placebo group. No effects were observed on HDL, TG or cardiovascular parameters or overall gut microbiota composition. Responders to LP intervention (> 5% LDL-C reduction) were characterized by higher BMI, pronounced TC reduction, higher abundance of fecal Roseburia and lower abundance of Oscillibacter . In conclusion, 12-week of L. plantarum intake moderately reduced LDL-C and TC as compared to placebo. LDL-C lowering efficacy of L. plantarum strains may potentially be dependent on individual difference in the gut microbiota. Trial registration: DRKS00006189, dated 17/12/2019.