Chromosomal-level genome assembly ofPopulus adenopoda

Abstract High-quality reference genomes for several species have promoted breeding and functional studies of poplar trees. By resequencing numerous accessions of these and closely related species, single nucleotide polymorphisms (SNPs) and small insertion/deletions (InDels) have been identified to assist in clarifying local adaptation and phenotypic diversification. A chromosome-level genome assembly for P. adenopoda was assembled based on Illumina and PacBio sequencing platforms, facilitated by Hi-C technology. The assembled genome size was about 383 Mb, with 99.70% of the contigs anchored to 19 pseudo-chromosomes, and a total of 33,505 protein-coding genes were annotated. This high-quality genome provided the genomic basis for the subsequent detection of various variants.