Why does Papilio bianor pupa Proton Magnetic Resonance Imaging show similar results in T1Weight image and T2Weight image?

Abstract To establish a noninvasive technique for visualizing Papilio bianor pupae, we devised parameters for Proton Magnetic Resonance Imaging at 9.4 T. We attempted five imaging modes: T 1 -weighted without fat suppression, T 1 -weighted with fat suppression, T 2 -weighted without fat suppression, T 2 -weighted with fat suppression, and T 2 -weighted with water-signal suppression. Among these, only T 2 -weighted imaging with water signal suppression mode was useful for detecting fat bodies located in the brain, abdominal fluid, and wing margins of the pupae. We believe that this method is useful for detecting fat bodies in butterfly pupae. In contrast, no significant differences were observed between these imaging methods, except for T 2 -weighted images with water signal suppression. We believe that further improvements are required to accommodate insect observations.