DNA Encoding Strategy for the Identification and Quantification of Poorly Reactive Target Metabolite Using Phenylboronic Acid-tethered Oligodeoxynucleotides

Information about poorly reactive metabolites, lactate and glucose could be encoded into DNA sequences. The sequential reaction steps, including H2O2 generation between the target metabolites and their corresponding oxidase, the reaction between phenylboronic acid-tethered oligonucleotide and H2O2, and biotinylation of product DNA allow the DNA encoding strategy to identify and quantify the target metabolites.