Effects of Ovariectomy on Cognitive Function and Estrogen Receptor Expression in Hippocampus of APP/PS1 Mice

Abstract INTRODUCTION: To investigate the effects of low estrogen environment on cognitive function and estrogen receptors in APP/PS1 mice. METHODS: A mouse model of cognitive dysfunction under low estrogen environment was established by removing ovaries, organelle morphology in CA1 area of mouse hippocampus was observed by transmission electron microscopy, Aβ deposition level in hippocampal area was detected by immunofluorescence and sulfur staining, serum estrogen level and oxidative stress index were detected by ELISA, and estrogen receptor α and β levels were determined by western blot. RESULTS: A low estrogen environment leads to increased Aβ deposition and decreased estrogen receptor alpha and beta levels in hippocampal tissue. DISCUSSION: This cognitive dysfunction may be related to abnormal Aβ deposition in a low estrogen environment.