Berberis vulgaris L. extract supplementation exerts regulatory effects on the lifespan and healthspan of Drosophila through its antioxidant activity

Abstract Worldwide aging population continues to increase, so the concept of healthy longevity medicine has become increasingly significant in modern society. Berberis vulgaris L. fruits serve as a functional food supplement with a high concentration of bioactive compounds, which offer numerous health-promoting benefits. The goal of this study was to investigate the geroprotective effect of Berberis vulgaris L. extract. Here we show that extract of Berberis vulgaris L. can, depending on concentrate, increase lifespan up to 6%, promote healthspan (stress resistance up to 35%, locomotor activity up to 25%, integrity of the intestinal barrier up to 12%, metabolic rate up to 5%) of Drosophila melanogaster ( in vitro ) and exhibits antioxidant (using red blood cell tests) and antiglycation activity (using glycation of bovine serum albumin) ( in vitro ). In addition to this, the extract does not exhibit cytotoxic properties in vitro , unlike the well-known polyphenolic compound quercetin. qRT-PCR revealed the involvement of metabolic, heat shock response and lipid metabolism genes in the observed effects.