Time-deterministic cryo-optical microscopy
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Abstract We developed a technique to rapidly freeze biological systems during observation under an optical microscope, which allows for detailed optical observation at a precise time in the biological dynamics at high spatial resolution and quantifiability under low-temperature conditions. Our study further found that ion distributions and molecular conformations can be fixed in space and time, demonstrated by visualization of calcium waves rapid-frozen at an instant using fluorescent ion indicators. These results show that time-deterministic cryo-optical microscopy can suspend cellular dynamics while maintaining molecular and ionic states, allowing us to view cellular dynamics in a way that has not been possible before. One-Sentence Summary Rapid freezing of dynamic biological systems during optical observation presents time information to the fixed samples
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关键词
time-deterministic,cryo-optical
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