Physical Sensors: Fluorescence Sensors

Fluorescence analysis is a powerful method for biochemical analyzes and medical diagnostic procedures. Various detection methods have been employed to detect fluorescence with high sensitivity. This chapter introduces the fluorescence principle and various detection methods and then explains the structures and operating principles of fluorescence microscopes and fluorescence spectrometers. A compact fluorescence sensor with high sensitivity and selectivity is required, and an on-chip fluorescence sensor incorporating a semiconductor process has been described. Finally, the structure, principle, and application of the filter-free fluorescence sensor are discussed.