Effective Cellulase Production by Trichoderma reesei Using Solid and Liquid Inducers Mixture with Varying Ratios

Cellulase serves as a pivotal enzymatic catalyst in the bioconversion of lignocellulosic biomass into fermentable sugars and subsequent bioproducts. However, its high cost poses a significant challenge, making the overall lignocellulosic biorefinery process economically burdensome. To mitigate this issue and enhance cost-effectiveness, it becomes imperative to employ in-house built and highly potent cellulases. This study focused on effective cellulase production by Trichoderma reesei RUT C30 and PB3 strains using solid and liquid inducers mixture with varying ratios. By optimizing the SLI mixture at a ratio of 1:5 (w/w), we successfully attained maximum cellulase production and productivity, reaching 29.5 FPU/mL and 204.8 FPU/L/h, respectively. Furthermore, this strategy yielded comparatively elevated levels of cellobiase and xylanase production, achieving 69.6 CBU/mL and 250.1 IU/mL, respectively, in contrast to the control process utilizing either solid or liquid inducers. Ultimately, hydrolysis of 10% (w/v) H 2 SO 4 -pretreated corn stover (HPCS) utilizing a crude enzyme dosage of 15 FPU/g-HPCS liberated 45.5 g/L of glucose and 18.2 g/L of xylose, underscoring the immense potential of this methodology in alleviating the cost burden associated with lignocellulosic biorefinery.