Studying the photothermal activation of polydopamine-shelled, phase-change emulsion droplets into microbubbles using small- and ultra-small-angle neutron scattering

Polydopamine-shelled perfluorocarbon (PDA/PFC) emulsion droplets are promising candidates for medical imaging and drug delivery applications. This study investigates their phase transition into microbubbles under nearinfrared (NIR) illumination in situ using small- and ultra -small -angle neutron scattering (SANS and USANS) and contrast variation techniques. Supported by optical microscopy, thermogravimetric analysis, and ultrasound imaging, SANS and USANS results reveal rapid phase transition rates upon NIR illumination, dependent on PFC content and droplet size distribution. Specifically, perfluoropentane droplets rapidly transform into bubbles upon NIR irradiation, whereas perfluorohexane droplets exhibit greater resistance to phase change (bulk boiling points = 30 degrees C and 60 degrees C, respectively). Furthermore, smaller emulsion droplets with unimodal distribution resist NIR-triggered phase changes better than their bimodal counterparts. This observation is attributable to the lower boiling points of large emulsion droplets (lower Laplace pressure than smaller droplets) and the faster photothermal heating rates due to their thicker polydopamine shells. The insights gained from these techniques are crucial for designing phase -change emulsions activated by NIR for photothermal therapies and controlled drug delivery.