Development and validation of a new visible spectrophotometric method for determination of fluconazole in the presence of some essential oils

This study aimed to develop and validate a visible spectrophotometry analysis method for fluconazole quantification in the presence of some essential oils (cinnamon, oregano and clove essential oil), by forming a coloured complex with Cu2+ ion. For the spectrophotometric method validation the spectra of the fluconazole-Cu(II) complex in the presence of volatile oils were analysed and the wavelengths corresponding to the maximum absorbance values of the complex were determined. Evaluating the precision, linearity, accuracy and robustness of the elaborated visible spectrophotometric method, the obtained values of the specific parameters fell within the limits provided by the standards in force. Linearity was very good for the following fluconazole concentration ranges: 0.268 -2.68 mg/mL for oregano essential oil, 0.2073 -2.073 mg/mL for cinnamon essential oil and 0.2317 -2.317 mg/mL in the case of clove essential oil. The proposed visible spectrophotometric method has been successfully applied for the assay of fluconazole in clove, cinnamon and oregano essential oils.