Bionanomaterial composed of Bradyrhizobium japonicum and graphene oxide for determination of mercury in water and fruit juice samples

In the present work, the synthesis of a novel hybrid bionanomaterial formed by the bacteria Bradyrhizobium japonicum (BJ) and the graphene oxide (GO) nanomaterial implemented in the miniaturized dispersive microsolid phase extraction technique (D-mu-SPE) was reported for the first time for the preconcentration and determination of Hg in water and fruit juice samples after a multivariate optimization. Selective Hg extraction was achieved by forming a complex with ammonium pyrrolidinedithiocarbamate (APDC) reagent and a multivariate study was performed to optimize the experimental conditions. The optimal Hg extraction conditions for 25 mL of sample were 3 mg of BJ@GO, NaNO3 at 1% (w/v), 6 min of vortex agitation and 9 min of centrifugation. For back extraction, 500 mu L of 14 mol L-1 HNO3, 6 min of vortex agitation and 6 min of centrifugation were used followed by detection by cold vapor atomic fluorescence spectrophotometry (CV-AFS). Under optimized experimental conditions, an extraction efficiency of 98%, a detection limit of 0.08 mu g L-1, a relative standard deviation of 3.5% (at 1 mu g L-1 of Hg, n = 10), and a linear range of 0.26 - 4.0 mu g L-1 were obtained. In addition, the sustainability of the method developed through the use of the Analytical GREEnness calculator (AGREE) software was evaluated, obtaining a greenness degree of 0.59. This work reports the first application of BJ@GO along with the D-mu-SPE technique for trace Hg determination water and fruit juice samples. In this way, this research work allows the exact and precise determination of low Hg concentrations in water and juice samples, which allows the evaluation of the contamination degree by this toxic metal in beverages widely consumed by the population.