Identifying compositional and density changes across the Murine and Human Dentin-enamel Junction

Human and mouse incisors are both primarily composed of dentin and enamel which meet at an interface called the dentin-enamel junction (DEJ). However, incisors in the two species have very different growth patterns, structures, and loading requirements. Since the DEJ is responsible for minimizing cracking at this at-risk interface between mechanically dissimilar dentin and enamel, its structure is expected to be significantly different between humans and mice. Here, strucutral and compositional gradients across human and murine incisors DEJs were measured via microcomputed tomography and Raman spectroscopy. Density gradients across the DEJ were significantly larger in humans compared to murine teeth likely due to the larger size of the mantle dentin. Multiple gradients in mineral content and crystallinity were found at the murine DEJ while the human DEJ only exhibited gradients in mineral content. Models predicting the modulus across the DEJ according to compositional results show that mineral crystallinity is critical in regulating the mechanical gradient across the murine DEJ. Together these results show the multiple ways in which the DEJ can adapt to variations in loading environment. ### Competing Interest Statement The authors have declared no competing interest.