Novel Pickering Emulsion Stabilized by Glycosylated Whey Protein Isolate: Characterization, Stability, and Curcumin Bioaccessibility

Pickering emulsions prepared from protein-polysaccharide complexes have attracted increasing attention. In this study, whey protein isolates (WPI) were modified with oligochitosan using transglutaminase (TGase)-type to fabricate Pickering emulsions, and loaded with curcumin. The curcumin/protein ratio of 1:25 and oil phase fraction (phi = 17 %) are the most optimal condition for emulsions stabilization, and particle size of glycosylated WPI emulsion was 31.70 mu m. Glycosylated WPI emulsion had the highest encapsulation efficiency (96.64 %) of curcumin. Microstructure analysis showed that glycosylated WPI had small droplets covered by dense interface layers. The modified WPI emulsions exhibited optimal emulsifying properties and emulsion stability, which effectively inhibited the premature water-oil stratification in emulsion. In vitro digestion results showed that WPI-oligochitosan complexes enhanced curcumin bioaccessibility (40.34 %). The antioxidant activity of glycosylated WPI emulsions was significantly increased. The results of this study provide helpful references for applying glycosylated WPI-stabilized Pickering emulsions, which can be used as transport carriers of curcumin.