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    • Gut Microbiota Modulation, Prebiotic and Bioactive Characteristics of Date Pomace Polysaccharides Extracted by Microwave-Assisted Deep Eutectic Solvent.

    Gafar Babatunde Bamigbade,Athira Jayasree Subhash,Basel Al-Ramadi,Afaf Kamal-Eldin,Ren-You Gan,Shao Quan Liu,Mutamed Ayyash

    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES（2024）

    United Arab Emirates Univ UAEU | ASTAR | Natl Univ Singapore

    Cited 1|Views33
    Abstract
    This study investigated the characteristics of polysaccharides from date pomace using microwave-assisted deep eutectic solvents. The impact on the gut microbiota and probiotics growth was examined in vitro. The study also examined its antioxidant properties, ability to inhibit enzymes linked to diabetes and high blood pressure, impact on cell growth, and physical properties. The isolated MPS had an average molecular weight of 8073.38 kDa and contained mannose, galacturonic acid, galactose, glucose, and fructose in specific proportions. At a concentration of 1000 mg/L, MPS showed strong antioxidant activity, with significant scavenging rates in various tests such as DPPH (57.0 ± 1.05 %) and ABTS (66.4 ± 2.48 %). MPS displayed 77 %, 80 %, and 43 % inhibition for α-amylase, α-glucosidase, and ACE-inhibition, respectively. MPS displayed significant antiproliferative effects, achieving 100 % and 99 % inhibition against Caco-2 and MCF-7 cells at 2500 mg/L, respectively. MPS showed broad-spectrum antibacterial properties against both Gram-positive and Gram-negative foodborne bacteria. Gemmiger formicilis, Blautia species, Collinsella aerofaciens, and Bifidobacterium longum showed strong positive correlations, suggesting increased SCFA production. Network analysis indicated species correlations, with 86 % showing negative correlations with Escherichia and Enterococcus saccharolyticus. MPS was abundant in Firmicutes, Actinobacteria, and Proteobacteria phyla. Date pomace could serve as a dietary fiber source, promoting better health.
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    Date pomace,Prebiotics,Broad-spectrum,Colonic microbiota,Metabolites
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    【要点】：本文研究了使用微波辅助共晶溶剂从日期果渣中提取的多糖特性，体外研究了其对肠道微生物群和益生菌生长的影响，并考察了其抗氧化、抑制糖尿病和高血压相关酶、细胞增殖以及对食物细菌的广谱抗菌性能。
    

    【方法】：研究采用微波辅助共晶溶剂法提取日期果渣中的多糖，并通过体外实验分析其对肠道微生物群和益生菌的影响。
    

    【实验】：实验结果显示，分离出的MPS具有8073.38 kDa的平均分子质量，含有特定比例的甘露糖、半乳糖酸、半乳糖、葡萄糖和果糖。在1000 mg/L的浓度下，MPS表现出强烈的抗氧化活性，DPPH和ABTS自由基清除率分别为57.0%±1.05%和66.4%±2.48%。MPS对α-淀粉酶、α-葡萄糖苷酶和ACE抑制的抑制率分别为77%、80%和43%。在2500 mg/L的浓度下，MPS对Caco-2和MCF-7细胞表现出显著的抑制效果，抑制率分别为100%和99%。MPS对食品细菌（包括革兰氏阳性菌和革兰氏阴性菌）表现出广谱抗菌性能。网络分析显示，与Escherichia和Enterococcus saccharolyticus的86%物种呈负相关，而Gemmiger formicilis、Blautia species、Collinsella aerofaciens和Bifidobacterium longum与SCFA产生呈强正相关。MPS在拟杆菌门、放线菌门和变形菌门中含量丰富。日期果渣可作为膳食纤维来源，促进更好的健康。