Time-Lapse Imaging of Mouse Brain Through Intact Skull Using a Label-Free Computational Conjugate Adaptive Optical Microscopy

Yongwoo Kwon,Seokchan Yoon,Jin Hee Hong,Sungsam Kang,Hojun Lee,Wonshik Choi

2022 Conference on Lasers and Electro-Optics Pacific Rim (CLEO-PR)（2022）

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摘要

We implemented a computational conjugate adaptive optical microscopy system using a $1.3-\mu \mathrm{m}$ wavelength laser and conducted in vivo imaging of a 3- to 10-week-old mouse brain with an intact skull and observed myelination process.

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关键词

Light Microscopy,Mouse Brain,Adaptive Optics,Intact Skull,Alzheimer’s Disease,Transformation Matrix,Nonlinear Optical,Second Harmonic Generation,Cortical Layers,Cranial Window,Label-free Imaging,Second Harmonic Generation Signal,Growing Research Interest,Attenuation Length,Need For Labeling

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