Novel Drug Therapy of Acute Hepatic Failure Induced in Rats by a Combination of Tadalafil and Lepidium Sativum

Background Hepatocyte death and a systemic inflammatory response are the outcome of a complex chain of events mediated by numerous inflammatory cells and chemical mediators. The point of this study was to find out if tadalafil and/or Lepidium sativum ( L. sativum ) could help people who have been exposed to carbon tetrachloride (CCL 4 ) and are experiencing acute moderate liver failure. This was especially true when the two were used together. Method and materials To cause mild liver failure 24 h before sacrifice, a single oral dosage of CCL 4 (2.5 mL/kg b.w.) (50% in olive oil) was utilized. Furthermore, immunohistochemical expression of nuclear factor kappa B (NF-κB) as well as histological abnormalities were performed on liver tissue. Results The results showed that tadalafil and/or L. sativum , especially in combination, performed well to cure acute mild liver failure caused by CCL 4 . This was demonstrated by a decrease in NF-κB expression in the liver tissue and an improvement in organ damage markers observed in the blood and liver tissues. Furthermore, such therapy reduced interleukin1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels in the liver tissue. It’s worth noting that the tested combination resulted in greater liver improvement. Conclusions According to the findings, tadalafil and L. sativum , particularly in combination, have the ability to protect the liver from the negative effects of CCL 4 exposure. Because of its capacity to improve liver function, restore redox equilibrium, and decrease inflammatory mediators, it is a prospective option for mitigating the negative effects of common environmental pollutants such as CCL 4 .