Wide detection range for non-enzymatic glucose monitoring by utilizing LDHs-coated CuO nanowires in biological media

Inorganic nanostructured materials show significant potential in improving diabetes treatments by serving as glucose-sensing elements that eliminate the requirement for expensive enzymatic agents. However, most non-enzymatic sensors suffer from limited selectivity and reliability, which compromise their suitability for practical applications to body fluids. In this study, we developed a novel non-enzymatic glucose sensor that exhibits a remarkable selectivity and high detection range (<8 mM) in real human serum. The sensor operated effectively across a wide range of glucose concentrations, with the ability to detect glucose levels of up to 8 mM in human serum. Critically, the sensor demonstrated a significant resistance to interference from other substances, which is a common challenge in biological sensing. Rigorous testing confirmed the precision of the sensor, as evidenced by its impressively low relative standard deviation (RSD) of less than 1.56 %, thereby highlighting its consistent reliability for potential clinical applications. Overall, the CuO nanowires (NWs)-NiFe layered double hydroxides (LDHs) sensor is significantly promising as a reliable, highly reversible, and cost-effective solution for glucose detection and continuous glucose monitoring systems.