Optimization of culture conditions of newly isolated cyanobacteria Nostoc BGLR 1 for nutraceutical formulation

Euryhaline cyanobacteria are autotrophic microbes that can be suitable for use in the development of nutraceutical formulation because of their high metabolic activity, resistance to euryhaline environments, ability to multiply quickly, and capacity to accumulate the economically important bioactive chemicals. The objective of this study was to characterize Nostoc BGLR1 for the production of nutraceutical compounds like carbohydrates, proteins, lipids, carotenoids, flavonoids, phenols, astaxanthin, and phycocyanin, as well as to standardize physio-chemical parameters such as pH (7.5-11.5), temperature (20-40 degrees C), light intensity (4000-8000 lux), growth period (20-40 days), and inoculum concentration (1-10%). Following optimization, the contents of Nostoc BGLR1 viz. biomass, chlorophyll a, chlorophyll b, total chlorophyll, carbohydrate, protein, and lipid augmented by 28,708.03%, 651.9%, 410.34%, 283.98%, 656.25%, 164.6%, and 860%, respectively. Twelve therapeutic compounds, including 2 acetoxyisobutyryl chloride, cyclohexasiloxane, dodecamethyl, caryophyllene, and cycloheptasiloxane tetradecamethyl, were found in the study by gas chromatography-mass spectrometry. BGLR1 also contained 6 ethyl 3-octyl butyl ester, 9,12-octadecadienoic acid, 9-octadecenoic acid (Z), methyl ester, 1-monolinoleoylglycerol trimethylsilyl ether, phytol, and methyl stearate. With the use of 16S rRNA gene sequencing, it was determined that BGLR1 belongs to the genus Nostoc (GenBank accession number OQ600797.1). Nostoc BGLR1 cyanobacteria is a potentially useful source for nutraceutical formulation.