Coumarin Based Fluorescent Probe Synthesized by One-step Reaction for Sensitive Detection of Hydrazine and Its Application

Abstract A coumarin derivative fluorescent probe 3-(2-naphthyl)-7-acetoxycoumarin (TTAM) for hydrazine detection was successfully designed and synthesized. Hydrogen spectroscopy (1H NMR) and high-resolution mass spectrometry (HRMS) showed that the nucleophilic substitution occurred between hydrazine and the acyl oxide group of TTAM led to the probe fluorescence intensity increasing significantly and a remarkable color change which could be distinguished by naked-eye. The reaction could be finished in 120 s, and the detection limit is only 3.1×10-8 M. Moreover, a simple test paper was successfully developed that could be applied for rapid detection of hydrazine in water samples.