Profile of cross-reactivity to common pollen allergens in Northwest China based on Component Resolved Diagnosis

Abstract The prevalence of allergic diseases such as Allergic Rhinitis and Asthma is steadily increasing globally, with pollen allergy being one of the most significant sensitizing factors. However, the cross-reactivity of different pollen allergies remains unclear, posing challenges in the diagnosis and treatment of individuals with multiple sensitivities. In this study, the Component Resolved Diagnosis technique was employed to simultaneously measure the specific IgE concentrations of 52 patients against Art and its components (Art v 1), Phl p and its components (Phl p 1/4/5/6/7/12), Bet v and its components (Bet v 1/2), Amb a and its component (Amb a 1), and Amb p. Additionally, sIgE inhibition tests were conducted for Art v, Phl p, and Bet v. Among Art v -positive patients, 64.6% showed positivity for Art v 1. In Phl p-positive patients, Phl p 12 had the highest positivity rate (75.0%). Among Bet v-positive patients, 75.6% exhibited positivity for Bet v 2, whereas for Amb a and Amb p-positive patients, 23.7% and 29.0% respectively showed positivity for Amb a 1. The sIgE inhibition assays results revealed that Art v had inhibition rates greater than 73.2% against Phl p and its component Phl p 12, as well as Bet v and its component Bet v 2. Simultaneously, Phl p had inhibition rates of 79.20% to 89.87% against Phl p 12 and Bet v. Bet v showed inhibition rates ranging from 11.9% to 59.8% against Phl p and Art v, with a better inhibition rate (76.80%) against Phl p 12. In conclusion, Art v 1 is identified as the principal component of Art v. The profilin proteins of Phl p and Bet v (Phl p 12 and Bet v 2), are implicated as potential cross-reactive elements contributing to polysensitization in patients with respiratory allergies in the Northwest region of China. This cross-reactivity leads to a shared sensitization mechanism among pollen allergens such as Art v, Phl p, and Bet v.