Sustainable strategy for enhancing growth of marine diatom and lipid production using RO and AC spent water

Sustainable cultivation strategies is a prerequisite for algal biorefineries targeting on reducing water and energy footprint. Thus, in this study, spent waters from the air conditioning unit (SP1), the water purifier/RO unit (SP2) and from the condenser tube of distillation unit (SP3) was filtered, autoclaved and reused in combination with artificial seawater- f/2 (ASW) media for cultivating oleaginous diatoms, Chaetoceros gracilis and Thalassiosira weissflogii and examine its impact on the growth and lipid production. Both strains showed a sharp rise in cell numbers in the test culture setups supplemented with 50