How do non-halophyte locust trees thrive in temperate coastal regions: A study of salinity and multiple environmental factors on water uptake patterns

Understanding plant water use patterns is crucial for comprehending the dynamics of the soil-plant-atmosphere continuum and evaluating the adaptability of plants across diverse ecosystems. However, there remains a gap in our comprehension of non-halophyte plants' water uptake patterns and driving factors in temperate coastal regions. For this reason, we used locust trees (a widely planted non-halophyte tree species in northern China) as a study subject. We collected water isotope data (delta H-2 and delta O-18) for locust trees xylem and soil over two consecutive growing seasons. The MixSIAR model was used along with five distinct sets of input data (single isotopes, uncorrected dual isotopes, and corrected dual isotopes incorporating delta H-2 data obtained by soil water line or cryogenic vacuum distillation methods) to infer water utilization patterns. The results indicated that locust trees primarily absorb shallow soil water (0-20 cm, 29.4% +/- 16.9%) and deep soil water (120-180 cm, 24.7% +/- 5.8%). Pearson's correlation analysis revealed the key driving factors behind water uptake patterns were vegetation transpiration and soil salinity. Remarkably, the build up of salts in the lower soil layer (60-120 cm) hinders the absorption of water by plants. To prevent high salt concentrations from affecting water uptake in non-halophyte plants, we recommend implementing sufficient irrigation from March to April each year to meet the water needs of plant growth and regulate the accumulation of salts in various soil layers. This study reveals the dynamic water utilization strategy of non-halophyte plants in temperate coastal regions, offering valuable information for water resources management.