Highly scalable multiplex serology by mass spectrometry analysis of beads

Abstract Serology testing is crucial for diagnostics and epidemiological surveillance. Traditional methods test one antigen at a time and are costly at large scale implementation. Here, we introduce a scalable, cost-effective, and multiplex serology immunoassay that uses mass cytometry to analyze polystyrene beads loaded with stable isotopes. Our approach can generate 18,840 unique barcoded beads and enables high-throughput detection of antibodies in small volumes of plasma. This scalable method allows simultaneous analysis of IgG and IgM levels across hundreds of samples, offering a significant advance to the multiplex capabilities of serology immunoassays.