Abstract 1563 A Novel FRET Biosensor for Visualizing Membrane-Mediated Dimerization of PIP5K

The phosphatidylinositol 4-phosphate 5-kinase (PIP5K) family of enzymes synthesize the majority of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) lipids in eukaryotes. Production of PI(4,5)P2 lipids is critical for the regulation of many cellular pathways including actin nucleation, endocytosis, Wnt signalosome formation, and ion channel gating. Despite the importance of PIP5K activity in eukaryotic cells, there remain open questions concerning the mechanisms that control PIP5K membrane localization and activity.