In vitro Anticancer Efficacy of Hydroalcoholic Extract from Crateva religiosa G.Forst. Bark on Human Ovarian Cancer Cells

Introduction Medical research on cancer treatment is getting worldwide attention in the present day. The usage of plant sources for cancer treatment getting much consideration because of their low toxic nature, easy availability, and with fewer side effects. This study illustrates the anticancer potential of Crateva religiosa G. Forst. extract against the human ovarian cancer cells (PA-1). Methods Cell viability was determined using MTT assay and AO/EtBr staining techniques were used to evaluate the cell death and anticancer activity. Additionally, antioxidant activities such as hydroxyl radical scavenging, DPPH, FRAP, and ABTS+․ were carried out. Moreover, the phytochemical profiling and their quantification were also performed. Results The hydroalcoholic bark extract of C. religiosa has a strong anticancer prospective against PA-1 with an IC50 value of 33.27 μg/ml. The antioxidant potential of the extract showed potent activity on various antioxidants. The phytochemical profiling of the extract showed the presence of various phytochemicals and the quantification indicated the presence of a total phenol content of 2.1 ± 0.09 mg GAE/g, total flavonoid content of 1.706 ± 0.01 mg CE/g, total alkaloid content of 1.237 ± 0.03 mg AE/g, and total terpenoid content of 1.004 ± 0.06 mg UAE/g. Conclusion The current findings of the study suggest the role of C. religiosa bark against human ovarian cancer cells (PA-1) and could be a potential drug source for the discovery of anticancer agents.