FIGURE 3 from MARCH2, a Novel Oncogene-regulated SNAIL E3 Ligase, Suppresses Triple-negative Breast Cancer Metastases

MARCH2 is a novel PTK6-regulated SNAIL E3 ligase. A, Schema of siRNA screening strategy used to identify PTK6-regulated SNAIL E3 ligases. B, MARCH2 siRNA (SMART Pool) identified as top candidate siRNA that restores SNAIL expression in P21d-treated MDA-MB-231/SNAIL- Luciferase cells by Western analysis. C, MARCH2 siRNA (SMART POOL or individual) prevents P21d-induced SNAIL downregulation in MDA-MB-231 cells.