YB-1 expression analysis in the developing mouse eye by immunohistochemistry

Abstract Objective: Cold shock proteins such as YB-1 (ybx1) function in the regulation of transcription, mRNA stability, and translation. Consequently, YB-1 contributes to differentiation, stress responses and oncogenesis. Eye development is a complex process involving the differentiation of a signifiant number of cell-types with distinct functions. Additionally, the adult eye is exposed to UV-radiation causing significant oxidative stress. We therefore hypothesized that YB-1 plays a role in eye development as well as stress defence. As a first step to understand YB-1 function in this context, we analyzed its expression in the developing and adult mouse eye by immunohistochemistry. Results: Expression of the YB-1 protein in the developing mouse eye at stages (E12, E15 and E18) and in adult eyes (P14) was detected in all retinal cells and in cells of the cornea and the lens epithelium at all stages investigated. These findings support a significant function of YB-1 in the eye, may be related to development and differentiation.