Figure S3 from Mass Spectrometry–Based Proteomics Identifies Serpin B9 as a Key Protein in Promoting Bone Metastases in Lung Cancer

SB9 is a key protein in promoting BM and bone-related adverse events (SRE) in LC A: Fracture frequency of the indicated mice (n = 10/group) from indicated experimental group. B: The incidence of lung, brain and other organ metastases in indicated groups from immunocompetent C57BL/6 mice with IC injection. *P<0.05; **P<0.01; ***P<0.001; ns: not significant; P values were obtained by Two-sides with chi-square (and Fisher's exact) tests. C-D: Cell clone formation assay. There was no difference in cell clone formation between LLC1-BM3-sr-ctrl vs LLC1-BM3-shSB9 and LLC1-Pa-sr-ctrl vs LLC1-Pa-OE-SB9 groups; hole plate photo(B); number of clone formation(B) (sr-ctrl：scramble control; OE:overexpression). E: Ki67 mRNA levels in LLC1 derivative cell lines.There was no difference in Ki67 mRNA levels between LLC1-BM3-sr-ctrl vs LLC1-BM3-shSB9 and LLC1-Pa-sr-ctrl vs LLC1-Pa-OE-SB9 groups. F: Flow cytometry analysis the proportion of mcherry positive cells in LLC1-BM3-OVA and LLC1-BM3-shSB9-OVA cells. G: The incidence of lung, brain and other organ metastases in indicated groups from immunodeficient BABL/C nude mice with CA injection. *P<0.05; **P<0.01; ***P<0.001; ns: not significant; P values were obtained by Two-sides with chi-square (and Fisher's exact) tests.